Dr. Anurag Payasi
Head Scientist | Cell Culture & Microbial Biotechnology
Postdoc
"Identification and development of potential Antibiotic Resistance Breakers (ARBs) can be used as an effective strategy to preserve the life of existing antibiotics"
Years at VMRC
Publications
Awards & Honors
Antimicrobial resistance poses a global health crisis wherein common and treatable infections have been becoming life-threatening. As bacterial resistance has emerged either through mutations in the genome or the acceptance of genes conferring resistance by horizontal gene transfer (HGT) against clinically relevant antibiotics, and multi-drug resistance strains have become widespread both in hospitals and the community, in all WHO regions, the development of new drugs has become of critical importance. However, the development of new antibiotics has become scientifically more complex, more expensive, and more time-consuming. The costs to develop a new drug is at least $1 billion and no new class has been found since 1987. Historical data show that, generally, only 1 out of 5 drugs that reach the initial phase of testing in humans will receive approval from the Food and Drug Administration.
One way to reposition drugs is to use them in combination with antibiotic resistance breaker. However, the mechanism by which antibiotic resistance breaker re-sensitize bacteria to antibiotic is not yet fully understood. So, my research interests focus on screening and understanding of the mechanism of action of potential antibiotic resistance breakers which can be used as a tool for breaking antibiotic resistance.
Antibiotic resistance has emerged as one of the biggest threats around the world. It has been rising steadily in every part of the globe, compromising our ability to treat infectious diseases. As there is a deficit of knowledge not only how antibiotics work, also how pathogens got resistant to essentially all of the available antibiotics? Hence, there is a need to understand the mechanisms of resistance development in order to control them. Although academia has already started working on the resistance mechanism, only a few pharmaceutical companies are working on it. Therefore, it becomes a need, as well as an opportunity to work on this concept and CCMB of VMRC, has become a pioneer in this field in India.
A solution to combat aminoglycoside and quinolone resistant Gram negative organisms.
International Journal of Current Research.7, 17006-17011
Chaudhary M, Payasi A. 2015.
Surveillance study for MRSA prevalence and susceptibility trends against mecA and vanA positive clinical isolates.
International Journal of advances in Pharmacy, Biology and Chemistry.4, 469-477
Chaudhary M, Payasi A. 2015.
Anti-inflammatory activity and COX 2 inhibition potential of Trois in Murine macrophage cell line.
Journal of Biochemical Technology. 6(1), 938-944
Chaudhary M, Payasi A. 2015.
Comparative in vitro activity of Mebatic against clinical isolates.
International Journal of Recent Scientific Research. 6, 3781-3785
Chaudhary M, Payasi A. 2015.
Comparative in vitro activity of Supime against Gram-negative clinical isolates.
International Journal of Pharmacy and Pharmaceutical Sciences. 7, 198-202
Chaudhary M, Payasi A. 2015.
Comparative in vitro activity of Tobracef against Gram-negative isolates.
International Journal of Recent Scientific Research. 6, 4102-4106
Chaudhary M, Payasi A. 2015.
Comparative in vitro activity of Zydotum against Gram-negative and Gram-positive clinical isolates.
IOSR Journal of Dental and Medical Sciences. 14, 75-78.
Chaudhary M, Payasi A. 2015.
Prevalence and molecular characterization of selected Metallo β-lactamase producing Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, and Pseudomonas aeruginosa and their antibiogram from India.
International Journal of Advances in Pharmacy, Biology, and Chemistry. 42, 303-312.
Chaudhary M, Payasi A. 2015.
Taming wall teichoic acid multidrug resistance in Gram-positive pathogens.
Journal of Chemical and Pharmaceutical Research. 7,18-23.
Chaudhary M, Payasi A. 2015.
Vancoplus kinetic study in resistant Staphylococcus aureus.
Journal of Pharmaceutical Sciences Review and Research. 31, 135-142.
Chaudhary M, Payasi A. 2015.
Microbial surveillance and susceptibility of gram-positive bacteria to antibiotic drugs.
IOSR Journal of Pharmacy and Biological Sciences. 10, 95-99.
Chaudhary M, Payasi A. 2015.
Evaluation of different drugs in down-regulation of efflux pump genes expression in methicillin-resistant Staphylococcus aureus strains.
Journal of Infectious Disease. 10, 184-189.
Chaudhary M, Patnaik SK, Payasi A. 2014.
Molecular characterization and in vitro susceptibilities of β-lactamase producing Escherichia coli, Klebsiella species, Acinetobacter baumannii, Pseudomonas aeruginosa and Staphylococcus aureus to CSE1034 and other β-lactams.
Asian Pacific Journal of Tropical Medicine. 7(Suppl 1), S217-S223.
Chaudhary M, Payasi A. 2014.
Inhibition of panton-valentine leukocidin toxin-induced neutrophil cell lysis by Vancoplus in resistant Staphylococcus aureus infections.
American Journal of Infectious Disease. 10, 154-163.
Chaudhary M, Patnaik SK, Payasi A. 2014
Evaluation of genotoxicity of CSE1034 through Ames and in vitro chromosomal aberration tests.
Tropical Journal of Pharmaceutical Research. 13, 527-532.
Chaudhary M, Payasi A. 2014.
Enhanced safety and protection of glutamate-induced hippocampal neuronal cells damage by Neurotol.
Journal of Biochemical Technology. 5, 782-787.
Chaudhary M, Patnaik SK, Payasi A. 2014.
Metronidazole resistance in anaerobes and mapping their susceptibility behavior.
American Journal of Infectious Disease. 10 (2), 56-63, 2014.
Chaudhary M, Payasi A. 2014.
Susceptibility of antibiotics to carbapenemase producers and means to overcome.
Journal of Pharmaceutical and Biological Sciences. 9;155-161.
Chaudhary M, Payasi A. 2014.
Inhibitory activity of Potentox against DNA gyrase of Enterobacteriaceae.
American Journal of Infectious Disease. 10;36-43.
Chaudhary M, Payasi A. 2014.
Aminoglycoside patterns and prevalence of the aminoglycoside-modifying enzymes in clinical isolates of Gram-negative pathogens.
Global Journal of Pharmacology. 8, 73-79.
Chaudhary M, Payasi A. 2014.
Effect of Tobracef on biofilms of aminoglycoside-resistant Pseudomonas aeruginosa.
ISOR Journal of Pharmacy and Biological Science. 9, 21-25.
Chaudhary M, Kumar S, Payasi A. 2014.
Prevalence, Genotyping of Escherichia coli and Pseudomonas aeruginosa clinical isolates for oxacillinase resistance and mapping susceptibility behavior.
Microbial and Biochemical Technology. 6, 063-067.
Chaudhary M, Payasi A. 2014.
The synergy of a novel antibiotic adjuvant entity against multidrug-resistant Enterobacteriaceae.
Americal Journal of Infectious Disease. 9, 204-213.
Chaudhary M, Kumar S, Payasi A. 2013.
Evaluation of genotoxicity of Potentox through Ames and in vitro chromosomal aberration test.
World Journal of Pharmacy and Pharmaceutical Sciences. 2, 5260-5270.
Chaudhary M, Kumar S, Payasi A. 2014.
The synergy of a novel antibiotic adjuvant entity against multi-drug resistant Methicillin-resistant Staphylococcus aureus and heterogeneous glycopeptide-intermediate Staphylococcus aureus.
Journal of Pharmacy Research. 7;781-786.
Chaudhary M, Kumar S, Bansal R, Payasi A. 2013.
Prevalence of heterogeneous glycopeptide intermediate resistance within methicillin-resistant Staphylococcus aureus clinical isolates.
American Journal of Infectious Disease. 9, 63-70.
Chaudhary M, Payasi A. 2013.
Evaluation of genotoxicity of Trois through Ames and in vitro chromosomal aberration tests.
Asia Pacific Journal of Tropical Biomedicine. 3, 902-906.
Chaudhary M, Payasi A. 2013.
Changing trends of commonly used intensive care unit antibiotics due to differential membrane permeability in resistant Escherichia coli collected in EASE program.
Journal of Microbial and Biochemical Technology. 5, 84-87.
Chaudhary M, Payasi A. 2013.
Kinetic studies of Metallo-β-lactamase NDM-1.
International Journal of Medicine and Medical Science. 46, 1293-1298.
Chaudhary M, Payasi A. 2013.
In vitro susceptibilities of 116 clinical isolates of Acinetobacter baumannii and Pseudomonas aeruginosa to conventional and new antibacterial agents.
The Journal of Infectious Disease. 112; 189-193.
Chaudhary M, Kumar S, Payasi A. 2013.
Inhibition of DNA relaxases by Elores to control spreading of the resistant gene through conjugation.
International Journal of Biochemistry. 108; 202-206.
Chaudhary M, Payasi A. 2013.
Prevalence and antimicrobial sensitivity of extended-spectrum β-lactamase producing Gram-negative bacteria from clinical settings in India from 2010-2012.
International Journal of Medicine and Medical Science. 46, 1212-1217.
Chaudhary M, Kumar S, Payasi A. 2013.
Clinical, microbial efficacy and tolerability of Elores, a novel antibiotic adjuvant entity in ESBL producing pathogens: a prospective randomized controlled clinical trial.
Journal of Pharmacy Research. 7, 275-280.
Chaudhary M, Payasi A. 2013.
A randomized, open-label, prospective, multicenter phase III clinical trial of Elores in the lower respiratory tract and urinary tract infections.
Journal of Pharmacy Research. 6, 409-413.
Chaudhary M, Payasi A. 2013.
Evaluation of membrane permeability potential of different drugs in multi-drug resistant carbapenemase producing clinical isolates.
Journal of Antimicrobials. 128;157-165.
Chaudhary M, Payasi A. 2013.
In vitro susceptibilities of clinical isolates of Escherichia coli and Klebsiella species to CSE1034 and other β-lactams.
The Journal of Antibiotics. 66; 495-497.
Chaudhary M, Kumar S, Payasi A. 2013.
Inhibition of in vitro mecA gene transfer among Indian clinical isolates of Staphylococcus aureus.
The Journal of Applied Biochemistry. 106,133-139.
Chaudhary M, Payasi A. 2013.
Elores: a new antibiotic adjuvant entity active against Metallo-β-lactamases producing organisms.
International Journal of Medicine and Medical Science., 46,1145-1150.
Chaudhary M, Kumar S, Payasi A. 2013.
Evaluation of genotoxicity of Sanovul through Ames and in vitro chromosomal aberration tests.
International Journal of Medicinal Plant. 105, 218-224.
Chaudhary M, Kumar S, Payasi A. 2013.
Inhibition of Metallo-β-lactamases by Elores.
Journal of Antimicrobials. 128, 177-182.
Chaudhary M, Payasi A. 2013.
Evaluation of genotoxicity of CVA1020 through Ames and in vitro chromosomal aberration tests.
British Journal of Pharmacology and Toxicology. 4, 95-100.
Chaudhary M, Kumar S, Payasi A. 2013.
Incidence, prevalence and control of multidrug resistant (MDR) carbapenamase producing Acinetobacter baumaniiin Indian intensive care units.
Journal of Pharmacy Research. 7, 175-180.
Chaudhary M, Kumar S, Payasi A. 2013.
Antimicrobial susceptibility patterns and molecular characterization of Klebsiella pneumoniae clinical isolates from north Indian patients.
International Journal of Medicine and Medical Science. 46, 1218-1224.
Chaudhary M, Kumar S, Payasi A. 2013.
Battling the methicillin-resistant Staphylococcus aureus biofilm challenge with Vancoplus.
Microbial Biochemical Technology. S10.
Chaudhary M, Payasi A. 2014.
Pseudomonas aeruginosa resistance caused by glycocalyx with Tobracef.
Microbial Biochemical Technology. 6, 207-211.
Chaudhary M, Bansal R, Payasi A. 2014
Disodium edetate as a conjugation inhibitor: a novel approach to curb the growing conjugal transfer of vanA in Gram-positive bacteria.
Journal of Pharmacy Research. 7, 70-74.
Chaudhary M, Kumar S, Payasi A. 2013.
Characterization of quinolone-resistant genes and control of qnrB transfer by Potentox in clinical isolates.
Journal of Pharmacy Research. 7; 62-69.
Chaudhary M, Kumar S, Payasi A. 2013.
Rising antimicrobial resistance of Pseudomonas aeruginosa isolated from clinical specimens in India.
Journal of Proteomics and Bioinformatics. 6, 005-009.
Chaudhary M, Kumar S, Payasi A. 2013.
A prospective study for antimicrobial susceptibility of Escherichia coli isolated from various clinical specimes in India.
Journal of Microbial and Biochemical Technology. 4,157-160.
Chaudhary M, Payasi A. 2013.
Molecular characterization and antimicrobial susceptibility study of Acinetobacter baumannii clinical isolates from the Middle East, African and Indian patients.
Journal of Proteomics and Bioinformatics. 5, 265-269.
Chaudhary M, Kumar S, Payasi A. 2012.
A novel approach to combat acquired multiple resistance in Escherichia coli by using EDTA as Efflux Pump Inhibitor.
Journal of Microbial and Biochemical Technology. 4, 126-130.
Chaudhary M, Kumar S, Payasi A. 2012.
Role of EDTA and CSE1034 in curli formation and biofilm eradication of Klebsiella pneumoniae: a comparison with other drugs.
The Journal of Antibiotics. 65, 631-633.
Chaudhary M, Payasi A. 2012.
Ethylenediaminetetraacetic acid: A non antibiotic adjuvant enhancing Pseudomonas aeruginosa susceptibility.
African Journal of Microbiology Research. 6, 6799-6804.
Chaudhary M, Kumar S, Payasi A. 2012.
Comparative antibacterial activity of a novel antibiotic: etimicin sulphate and other aminoglycosides.
World Journal of Microbiology and Biotechnology. 28(12), 3365-71.
Chaudhary M, Naidu GK, Kumar S, Payasi A. 2012.
Role of CSE1034 in bacterial lipids and polysaccharides involved in biofilm formation: a comparison with other drugs.
African Journal of Microbiology Research. 6, 6525-6531.
Chaudhary M, Payasi A. 2012.
Comparative efficacy of antibiotics in biofilms eradication formed by ESBL and ESBL producing micro-organisms.
International Journal of Drug Development Research. 4, 138-147.
Chaudhary M, Payasi A. 2012.
An alternative approach to increase antibiotic sensitivity of coagulase +ve and -ve Staphylococcus spp. in planktonic and sessile cells.
Journal of Pharmacy Research. 5, 316-320.
Chaudhary M, Payasi A. 2012
Sulbactomax prevents antimicrobial resistance development by inhibition of conjugal transfer of F plasmids.
International Journal of Drug Development Research. 4, 337-345.
Chaudhary M, Payasi A. 2012.
A comparative study of Sulbactomax versus Ceftriaxone and a beta-lactamase inhibitor and their effect on mutant prevention in ESBL producing organisms.
International Journal of Drug Development Research. 3; 366-371.
Chaudhary M, Kumar S, Payasi A. 2011.
Comparative safety evaluation of Potentox® Vs co-administration of and amikacin in healthy Albino rat.
International Journal of Drug Development Research. 3, 348-355.
Chaudhary M, Payasi A, Dwivedi VK. 2011.
Potentox reduces the biochemical and inflammatory response in osteomyelitis infections.
Asian Journal of Osteoporosis and Metabolic Disorders. 4, 26-36.
Dwivedi VK, Soni A, Payasi A, Ahmad A, Gupta SP, Chaudhary M. 2011.
Sub-acute toxicity study of a new aminoglycoside etimicin sulphate in Swiss albino mice.
The Journal of Toxicological Science. 35, 479-484.
Payasi A, Chaudhary M, Gupta A, Dwivedi VK. 2010.
Sub-acute toxicity studies of paracetamol infusion in Albino Wistar.
International Journal of Pharmaceutical Science and Drug Research. 2, 142-145.
Payasi A, Chaudhary M, Singh BM, Gupta A, Sehgal R. 2010.
Pharmacokinetic study of Sulbactomax.
The Journal of Toxicological Science. 35, 459-464.
Payasi A, Chaudhary M, Gupta A, Dwivedi VK, Bhatnagar A. 2010.
Acute dermal toxicity studies of Trois in Newzealand white rabbits.
International Journal of Drug Development and Research. 2, 310-313.
Payasi A, Gupta A, Chaudhary M, Sehgal R, Naithani V. 2010.
Acute dermal irritation studies of Trois in New Zealand white rabbits.
Life Science Bulletin. 7, 267-68.
Payasi A, Gupta A, Chaudhary M, Sehgal R, Naithani , Pathak N. 2010.
Sub-acute toxicity studies of paracetamol infusion in musculus mice.
International Journal of Drug Development and Research. 2, 157- 163.
Payasi A, Gupta A, Chaudhary M, Dwivedi VK, Singh BM, Shrivastava SM. 2010.
Improvement of the blood-brain barrier and antioxidant activities by immunox v drug in meningitis.
International Journal of Drug Development and Research. 2, 54-61.
Dwivedi VK, Ahmad A, Payasi A, Chaudhary M. 2010.
In vitro tissue penetration study of sulbactomax: a novel fixed-dose combination of ceftriaxone and sulbactam in rats.
International Journal of Drug Development and Research. 2, 141-147.
Dwivedi VK, Payasi A, Ahmad A, Bhatnagar A, Siddiqui MR, Tariq A, Chaudhary M. 2010.
Sub-acute toxicity of aceclofenac drug.
Iranian Journal of Toxicology. 2, 240-245.
Tamta A, Payasi A, Chaudhary M, Ahmad A,Dwivedi VK. 2009.
Comparative toxicity profile study of mebatic vs ofloxacin, ornidazole and metronidazole drugs in the rat model.
Asian Journal of Biochemistry. 5, 78-88.
Ahmad A, Chaudhary M, Soni A, Payasi A, Dwivedi VK. 2009. s
Garlic extract enhances the shelf life of ripe banana fruits.
International Journal of Food Science and Technology. 42, 303-311.
Sanwal GG, Payasi A. 2007.
Purification and characterization of pectate lyase from banana fruits.
Phytochemistry. 67, 861-869.
Payasi A, Misra PC, Sanwal GG. 2006.
Effect of phytohormones on pectate lyase during ripening of Musa acuminate.
Plant Physiology and Biochemistry. 42, 861-865.
Payasi A, Misra PC, Sanwal GG. 2004.
Pectate lyase activity during ripening of banana fruits.
Phytochem., 63; 243-248.
Payasi A, Sanwal GG. 2004.
Role of CSE1034 in Escherichia coli biofilm destruction.
journal of Microbial and Biochemical Technology. 5, 54-58.
Chaudhary M, Kumar S, Payasi A. 2013.
Inhibition of ATPase activity by Elores.
Journal of Antimicrobials. 128;183-188.
Chaudhary M, Payasi A. 2013.
- Post-doctoral research in 2009 at University of Federal, Pelotas Brazil
- Doctorate in 2006 in Biochemistry at Lucknow University, Lucknow
- Post-graduate in 2000 in Biochemistry at APS University, Rewa, MP, India
- Graduate in 1998 in life science at APS University, Rewa, MP, India
- Awarded for “Best productive employee ” of the Venus Medicine Research Centre in the year 2015.
- Got appreciation from “Chairman & Managing Director” Venus Remedies Limited for successful completion of 50 publications on Elores in 2014.
- Recipient of “Award for best performer of Venus medicine Research Centre 2013”.
- Established Cell Culture and Molecular biology Department in Venus Medicine Research Centre in the year 2011.
- Awarded for most innovative employee of the Venus Medicine Research Centre in the year 2011.
- Awarded silver medal for poster presentation in an International conference “Manthan” held in Venus Medicine Research Centre, Baddi from January 14-16, 2010.
- Being a founder member, Established Department of Biotechnology (2007) in JMIT, Radaur, Yamunanagar, Haryana.
- Recipient of CNPQ-TWAS Postdoctoral Fellowship 2007(FR 3240157219).
- Recipient of “Best Research Paper Award-2004” by Lucknow University, Lucknow, India.
- Payasi A, Ganguly K, Roy D, Sachdeva A, Chaudhary M, Chaudhary S, Aggarwal A. Poster number L0029 entitled “Pharmacodynamic Assessment of VRT001-C (Oral Ceftriaxone) vs. IV Ceftriaxone Against Escherichia coli in the Neutropenic Murine Thigh Infection Model” presented at the 29th ECCMID, the European Congress of Clinical Microbiology and Infectious Diseases, which took place in Amsterdam, Netherlands, 13 – 16 April 2019.
- Mandale P, Mir MA, Chaudhary S, Chaudhary M, Sood R, Patil NS, Narang S, Payasi A, Shameem M, et al. Poster number 1974 entitled “Ceftriaxone-Sulbactam-EDTA vs. Meropenem in PLEA (a Phase 3, Randomized, Double-blind Trial): Outcomes by Baseline MIC in Adults with Complicated Urinary Tract Infections or Acute Pyelonephritis” presented at IDWeeK-2018, October 3-7, 2018 at the Moscone Convention Center, in San Francisco, CA.
- Girotra R, Payasi A, Chaudhary M, Patil NS, Mir MA, Chaudhary S, Mandale P, et al. Poster number 1373 entitled “Activity of Ceftriaxone-Sulbactam-EDTA Against Multi-Drug Resistant A. baumannii, P. aeruginosa and Enterobacteriaceae Isolates (WHO Critical Priority Pathogens) Collected from Various Hospitals in India” presented at IDWeeK-2018, October 3-7, 2018 at the Moscone Convention Center, in San Francisco, CA
- Mir MA, Chaudhary S, Chaudhary M, Payasi A, Patil NS, Mandale P, Girotra R, Fatima N, et al. Poster number 1948 entitled “Ceftriaxone-Sulbactam-EDTA vs. Meropenem: Analysis of failed patients with assessment of MIC increases and changes in genotypic profile in PLEA (a Phase 3, Randomized, Double-blind Clinical Trial in Adults with Complicated Urinary Tract Infections or Acute Pyelonephritis)” presented at IDWeeK-2018, October 3-7, 2018 at the Moscone Convention Center, in San Francisco, CA.
- Chaudhary M, Payasi A, Patil S. Poster number 192 entitled “Antimicrobial Resistance in India - Genotypic Characterization and Exploring the Role of EDTA as an Antibiotic Resistance Breaker” presented at ASM/ESCMID conference on drug development to meet the challenges of antimicrobial resistance September 6-8, 2017 in Boston, MA.
- Awarded gold medal for poster presentation in an International conference “Molecular Pharmacology, Drug discovery and Nanopharmaceuticals” held in Chitkara College of Pharmacy, Chitkara University, Rajpura, Distt Patiala, Punjab. from March 27-28, 2015.
- Payasi A, Gupta A, Chaudhary M. Awarded silver medal for poster presentation in an International conference “Manthan” held in Venus Medicine Research Centre, Baddi from January 14-16, 2010.
- Payasi A (2007). Attended a workshop on immobilized Enzyme Technology and Biosensor organized by Department of Biotechnology, New Delhi, at Rohtak, Haryana, from Aug. 24 to Sep. 2.
- Payasi A, Misra PC, G. G. Sanwal (2005). Mg2+ requirement pectate lyase from banana fruits. Presented the poster in the 74th annual meeting of biochemical society Lucknow chapter, on Nov,7-10 at Central Drug Research Institute Lucknow.
- Payasi A, Misra PC, G. G. Sanwal (2003). Effect of phytohormones on Pectate lyase activity. Presented the poster in national conference on “Soil biodiversity and applied biology of the plant” on Nov, 8-10 at Lucknow University, Lucknow India.
- Participated in the 89th session of Indian Science Congress-2002 from January 3-7, 2002 at University of Lucknow, Lucknow in the section of Biochemistry, Biophysics, and Molecular Biology.
